ACLAR FILM EMBEDDING

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This method simultaneously offers transparency and thinness of the embedded blocks, absolute flatness of the sections, and easy removal of Epon from embedding molds. For a while, we believed that 2 mils is just not thick enough for applications in microscopy, and we started offering 7. Transfer learning based deep CNN for segmentation and detection of mitoses in breast cancer histopathological images. It furthers the University’s objective of excellence in research, scholarship, and education by publishing worldwide. This article was originally published in. However, the manufacturer of ACLAR films has discontinued the production of the 5 mil film and only offers the film in 2 and 7.

Sign in via your Institution Sign in. But good results are indeed possible with the 2 mil product as well. Email alerts New issue alert. Sign In or Create an Account. Citing articles via Web of Science 3. But if you were to try the thinner product, in addition to finding it easier to seal, you will be happily surprised to find out that the thinner version of the product is also much less expensive! One word of caution:

Sign In or Create an Account. One word of caution: Citing articles via Web of Science 3.

ACLAR Film 33C, 7.8 mils thick, 10 x 8″”

But good results are indeed possible with the 2 mil product as well. Although the 5 mil film sectioned quite acceptably, we are told that the 2 mil product sections even easier. Hajime Sawada, Michiyo Esaki; A simple flat embedding method for the correlative fiilm and electron microscopic immunocytochemistry, Journal of Electron MicroscopyVolume 47, Issue 5, 1 JanuaryPages —, https: You could not be signed in.

Most users should embeddiing in with their email address. For correlative light and electron microscopic immunocytochemistry, a simple method for flat embedding of cryostat sections in Epon was devised.

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ACLAR®–Fluoropolymer Films

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But now we have found that some users actually show a preference for the 2 mil thick product. Acclar learning based deep CNN for segmentation and detection of mitoses in breast cancer histopathological images.

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To purchase short term access, please sign in to your Oxford Academic account above. Maximum dimension is 10″. Because of the Cl content in the film, we would urge caution before using ACLAR film in conjunction with any EDS aclwr because of the large count rate that would be coming from the presence of Cl in the film. It can be applied to glass, such as a glass slide, without there being any real evidence for the presence of the film.

We can summarize some of the most desirable qualities of the ACLAR films, all of which are as true of the 2. This is a polychlorotrifluoroethylene copolymer and this particular composition results in a film with very excellent optical properties, as well as good thermal and chemical stability. And finally, the film shows no signs of deterioration even after long term exposure to UV. In general, we fil, recommend against use of this film when EDS is contemplated, unless of course there were no other alternatives which often times could be the case.

Resistance to osmium tetroxide: Email alerts New issue alert. But if you were to try the thinner product, in addition to finding it easier to seal, you will be happily surprised to find out that the thinner version of the product is also much less expensive!

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Sign in via your Institution Sign in. That is why it is often times called an “embedding” film, which is why we call the 2 mil product “new and improved”. This article was originally published in. Microscopy in with a renewed editorial board. The Epon block can be easily removed from slides by heating. Sign In Forgot password? Abstract For correlative light and electron microscopic immunocytochemistry, a simple method for flat embedding of cryostat sections in Epon was devised.

This article is also available for rental through DeepDyve. Oxford University Press is a department of the University of Oxford. Don’t have an account?

Don’t already have an Oxford Academic account? In the present technique tissue sections were attached to widely used silane-coated slides, processed, and embedded in Epon beneath non-adhesive transparent plastic films.

It also serves as an excellent moisture barrier. Receive exclusive offers and updates from Oxford Academic. Close mobile search navigation Article navigation.

Aclar Film Technical Data Sheet

ACLAR 33C film can be cut with a fairly ordinary scissors and reportedly, does not damage diamond knives during sectioning. The optical properties and UV transparency is essentially the same. Some comments specific to use in microscopy and histology laboratories The fluorinated-chlorinated polymers, because of the fluorinated aspect of the system, exhibit, in some respects almost PTFE-like properties, and therefore separates easily from all of the commonly used epoxy embedding formulations used in microscopy and histology.